- Mr Hart said Mr Bridgen would lose the party whip - meaning he will sit as an independent - while a formal investigation takes place.
- The MP is currently serving a five-day suspension from Parliament for breaching parliamentary rules on registering financial interests.
Andrew Bridgen has been suspended as a Conservative MP for spreading misinformation about Covid vaccination.
It comes after the North West Leicestershire MP posted a tweet that compared vaccines to the Holocaust.
Tory chief whip Simon Hart said the comments had "crossed a line" and caused great offence.
Prime Minister Rishi Sunak also condemned the remarks, calling the comparison "utterly unacceptable".
Mr Hart said Mr Bridgen would lose the party whip - meaning he will sit as an independent - while a formal investigation takes place.
"As a nation we should be very proud of what has been achieved through the vaccine programme," the chief whip added.
"The vaccine is the best defence against Covid that we have. Misinformation about the vaccine causes harm and costs lives."
The BBC has contacted Mr Bridgen for a comment.
The MP is currently serving a five-day suspension from Parliament for breaching parliamentary rules on registering financial interests.
In recent weeks, he has become increasingly vocal in his criticism of programmes to vaccinate people against Covid-19.
Posting a link to an article on vaccines earlier, he added: "As one consultant cardiologist said to me, this is the biggest crime against humanity since the Holocaust."
Karen Pollock, chief executive of the Holocaust Educational Trust, called the comments "highly irresponsible and wholly inappropriate".
Lord Mann, an independent peer and former Labour MP who advises the government on anti-Semitism, said Mr Bridgen should be barred from standing for the Tories at the next election.
"He cannot claim that he didn't realise the level of offence that his remarks cause," he added.
Conservative chairman Nadhim Zahawi, a former vaccines minister, said he was "proud" of the UK's response to the pandemic, and he was "appalled" by Mr Bridgen's remarks.
"Any comparison made to the Holocaust is completely inappropriate, belittling one of the greatest horrors ever committed by humanity," he added.
A spokesman for Labour leader Sir Keir Starmer said that suspending the whip had been "the right thing to do" and accused Mr Bridgen of "whipping up anti-vax conspiracy theories".
He said it was "regrettable" that the prime minister had not acted sooner to "slap down what he has been saying".
Asked whether he thought the MP's remarks were anti-Semitic, Sir Keir's spokesman said: "A one word reference to the Holocaust would not meet the definition of anti-Semitism, but it is unacceptable", adding that he would "defer to others who may be better placed to judge this".
Analysis by Rachel Schraer, Health and Disinformation reporter
Andrew Bridgen was previously critical of policies like lockdown and the idea of vaccine mandates - but he praised the development of the Covid vaccine, and tweeted proudly when he received his doses.
It wasn't until last autumn that he began to make increasingly baseless claims, including that the vaccines were killing many people and that the damage was being covered up.
At first, he began highlighting some real, but rare, instances of genuine vaccine injury and misinterpreting real data to suggest these cases were more common than the research suggests.
But in recent weeks, this rhetoric has ramped up.
Extensive independent research shows that Covid vaccines are extremely effective at preventing deaths and that serious side effects (including approximately 60 deaths in England and Wales) are rare, given the tens of millions of doses administered.