Wiper leader Kalonzo Musyoka said the move to remove the four IEBC commissioners is politically motivated and instigated.
"The persecution of the four commissioners is very sad. People are following a script," he said.
Kalonzo said the Justice and Legal Affairs Committee should be independent and stand out as a different House committee.
The Wiper leader hinted at demonstrations to protest the move.
"Kenyans would have wanted a peaceful Christmas. Why are they taking us to the streets," Kalonzo said.
Narc Kenya leader Martha Karua said there was a witchhunt in the removal of the commissioners.
"If we drop our vigilance, we will be in for a rude shock. We may have a ruthless dictator the region has ever seen," she said.
Karua alleged that Ruto is acting outside the law by not putting in place a panel for the selection of IEBC chair as Chebukati's term comes to an end.
He is instead directing his energies to amending the law to put in place a commission that suits him.
"The three top officials whose term is ending should be on leave," she added.
Azimio spokesman Makau Mutua said the opposition will not allow the removal of the four commissioners.
"We will ask our supporters shortly to stand with us to stop this," he said.
Mutua said JLAC lacks jurisdiction to investigate the conduct of the four.
He said the committee cannot interrogate the four basing the arguments on an affidavit sworn by Chebukati.
"The chairman has a script and wants to conclude the process quickly," he said.
"It's a slaughterhouse being prepared for the four commissioners."
