Security agencies have so far arrested 11 suspects linked to the chaos witnessed in Bomet and Kericho tea farms.
A statement on Tuesday by Interior CS Kithure Kindiki said the counties' security and intelligence teams had also closed in on other suspects who remain at large.
They include political leaders and businessmen, whom the CS said had incited the public to invade and destroy the tea farm.
He added that the leaders will be required to record statements before the respective county security and intelligence committees, in the coming days.
"Anyone found culpable will be prosecuted in accordance with the law. No one will be spared," Kindiki said.
He directed the security agencies in both counties to undertake their mandate without fear or favour.
The CS said the government was committed to facilitating a peaceful resolution to the disputes between the tea farm owners, workers and the county governments.
On Monday, residents torched nine green leaf plucking machines and trucks at Ekaterra Company in Kericho County.
After torching them, they drove one of the machines to Brooke trading centre and torched it by the roadside.
The incidents left five police officers, a Nation journalist and a youth injured.
This was an escalation to the dispute between the residents and multinational tea companies.
In Bomet, six police officers were attacked and injured a week ago by youths who were illegally harvesting tea at the James Finlay Tea Company farm.