Kenya's 200m record holder, Carvin Nkanata finally got a chance to feature Tuesday's heat at the Rio games but finished last as compatriot Mike Mokamba failed to start.
Nkatana's time of 21.43 was the slowest in all the eight heats.
The men's 200m race was won by LaShawn Merritt of United States in 20:15, while Christophe Lemaitre of France came second in 20.30.
Nkatana had received last-minute clearance to participate from the International Olympics Committee on Monday.
He had been locked out of the Olympics Village on August 4 for lack of an accreditation card.
He had only his Kenyan ID and American passport, but the two documents were initially rejected.
Nkatana's accreditation card is said to have been sent by the team management to his base in South Carolina.
With a record at 20.14 seconds, Nkatana was Kenya's top qualifier in the men's 200m.
Meanwhile, Mokamba's Did Not Start Status was due to an injury he picked in training.
"The doctor advised him not to run even though I
was not informed late, " team Kenya chef de mission said.
The heat was, however, won by Nery Brenes of Costa Rica in 20.20 while Churandy Martina of Netherlands was second in 20.29.
Brendon Rodney of Canada placed third
in 20.34 seconds.